女人张开腿让男人添,在线?亚洲?国产 欧美,嫩草乱码一区三区四区,特级做A爰片毛片免费69

Expert forum

首頁 專家論壇 標(biāo)本專家Cytometric characterization of circulating tumor cells captured by microfiltration and their correlation to the CellSearch(?) CTC test.
注冊(cè)

Cytometric characterization of circulating tumor cells captured by microfiltration and their correlation to the CellSearch(?) CTC test.

2017-12-08 06:57來源:原版作者:Adams DL

Recent studies reporting hundreds, to thousands, of circulating tumor cells (CTCs) in the blood of cancer patients have raised questions regarding the prevalence of CTCs, as enumerated by the CellSearch(®) CTC Test. Although CellSearch has been shown to consistently detect clinically relevant CTCs; the ability to only capture EpCAM positive cells has led to speculation that it captures limited subsets of CTCs. In contrast, alternative approaches to CTC isolation are often cited as capturing large numbers of CTCs from patient blood. Not surprisingly the number of cells isolated by alternative approaches show poor correlations when compared to CellSearch, even when accounting for EpCAM presence or absence. In an effort to address this discrepancy, we ran an exploratory method comparison study to characterize and compare the CTC subgroups captured from duplicate blood samples from 30 breast and prostate cancer patients using a microfiltration system (CellSieve?) and CellSearch. We then categorized the CellSieve Cytokeratin(CK)+/CD45-/DAPI+ cells into five morphologically distinct subpopulations for correlative analysis. Like other filtration techniques, CellSieve isolated greater numbers of CK+/CD45- cells than CellSearch. Furthermore, analysis showed low correlation between the total CK+/CD45- cells captured by these two assays, regardless of EpCAM presence. However, subgrouping of CK+/CD45-/DAPI+ cells based on distinct cytokeratin staining patterns and nuclear morphologies elucidated a subpopulation correlative to CellSearch. Using method comparison analyses, we identified a specific CTC morphology which is highly correlative between two distinct capture methods. These data suggests that although various morphologic CTCs with similar phenotypic expressions are present in the blood of cancer patients, the clinically relevant cells isolated by CellSearch can potentially be identified using non-EpCAM dependent isolation.

版權(quán)聲明:

本網(wǎng)站所有注明“來源:“陽普醫(yī)療”的文字、圖片和音視頻資料,版權(quán)均屬于陽普醫(yī)療所有,非經(jīng)授權(quán),任何媒體、網(wǎng)站或個(gè)人不得轉(zhuǎn)載,授權(quán)轉(zhuǎn)載時(shí)須注明

“來源:陽普醫(yī)療”。本網(wǎng)所有轉(zhuǎn)載文章系出于傳遞更多信息之目的,且明確注明來源和作者,不希望被轉(zhuǎn)載的媒體或個(gè)人可與我們聯(lián)系,我們將立即進(jìn)行刪除處理。

網(wǎng)友評(píng)論:

  • <center id="wvtgz"><tr id="wvtgz"><track id="wvtgz"></track></tr></center>
  • <ul id="wvtgz"></ul>

      
      
        1. 主站蜘蛛池模板: 方城县| 江陵县| 克拉玛依市| 南部县| 察隅县| 博白县| 临桂县| 乌拉特中旗| 上饶市| 襄汾县| 达州市| 内黄县| 广灵县| 岳阳县| 喀喇沁旗| 永仁县| 高安市| 东乡族自治县| 福贡县| 阿克| 内丘县| 辽源市| 东海县| 芦溪县| 新田县| 许昌市| 兴文县| 枣庄市| 南安市| 廉江市| 临西县| 玉田县| 政和县| 卢湾区| 陆河县| 潮安县| 济南市| 大足县| 阳信县| 刚察县| 新田县|